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Expert Lipidomics Solutions

The Yale Lipidomics Innovation Core (YLIC) provides a high-resolution window into biological systems. We specialize in identifying and quantifying complex lipid species with unparalleled precision, empowering researchers to uncover deep molecular insights and drive diagnostic innovation.

Service Details

S.01
Sample Preparation

We handle your samples with standardized, reproducible protocols from receipt to extraction. Samples processed in randomized batches if requested. When absolute quantification is requested, matched deuterated internal standard panels are spiked. When Additional Prep for Quantification is requested, Protein-normalized data is provided for downstream quantitative analysis.

Sample Preparation (Liquid Form)

•  For plasma, serum, urine, media, and other liquid biological samples.

•  MTBE-based or Bligh-Dyer/Folch-based biphasic extraction optimized for broad lipid recovery.

Sample Preparation (Cell Pellet)

•  For adherent or suspension cell pellets, including primary and cultured lines.

•  Cell lysis and lipid extraction in a single streamlined workflow.

Sample Preparation (Tissue)

•  For fresh-frozen or snap-frozen tissue specimens of any organ type.

•  Mechanical homogenization followed by optimized lipid extraction.

•  Wet-weight normalization included; minimum tissue requirements provided upon consultation.

Additional Prep for Quantification

•  Add-on service for projects requiring protein-based normalization.

S.02
Data Acquisition

High-resolution Orbitrap LC-MS/MS acquisition tailored to your analytical goals.

Data Acquisition for Identification Only

•  Untargeted, full-scan LC-MS/MS profiling on Thermo Orbitrap platforms.

•  Covers hundreds of lipid species across all major and minor lipid classes.

•  Ideal for discovery, biomarker screening, and hypothesis generation.

Data Acquisition for Identification and Quantification​

•  Full-scan with spiked internal standards in a single run.

•  Pairs broad lipid coverage with precise quantification of selected panels.

S.03
Data Processing

Automated pipelines paired with expert curation turn raw spectra into publication-ready results.

Data Processing for Identification Only

•  Automated spectral matching and lipid annotation against curated databases.

•  MS/MS-level confirmation for high-confidence identifications.

•  Deliverable: annotated lipid list in spreadsheet format.

Data Processing for Identification and Quantification

•  Full identification pipeline plus absolute or relative quantification.

•  Internal-standard normalization, batch correction, and QC filtering applied.

•  Deliverable: quantified lipid report, ready for statistical analysis.

S.04
Strategic Consulting

Expert guidance at every stage — from experimental design through manuscript submission.

Data Consulting (Per Hour)

•  Pre-submission consultation on study design, sample requirements, and statistical power.

•  Method Development for specific experimental requirements

•  Training for sample preparation, data processing and interpretation

•  Guidance on manuscript preparation, figure design, and methods sections for publication.

The Workflow

Step 01

Initial Inquiry

Submit a service request through PPMS to initiate the project consultation and feasibility review.

Step 02

Sample Preparation

Follow our optimized extraction protocols for your biological matrices to maintain sample integrity before shipping.

Step 03

Data Acquisition

High-resolution mass spectrometry using nano-spray chromatography for deep functional lipid profiling.

Step 04

Data Processing

Rigorous computational analysis, annotation, and quantification using our validated discovery pipelines.

Step 05

Insights Delivery

Final delivery of comprehensive data reports and a strategic consultation to discuss experimental findings.

Case Study: Deep Lipid Profiling in Neurodegenerative Research

A recent collaboration identifying 400+ unique lipid species in human brain tissue samples. Our core provided high-resolution mass spectrometry and comprehensive profiling to reveal critical metabolic markers associated with cellular aging.

Start Your Project

Request a consultation or receive a detailed quotation tailored to your specific research requirements. Our team is ready to support your lipidomics journey.

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